Home
HelpTest Code SPPS Stiff-Person Spectrum Disorders Evaluation, including Progressive Encephalomyelitis with Rigidity and Myoclonus, Serum
Additional Codes
Epic EAP: LAB11192
Epic Description: STIFF-PERSON/PERM EVAL, S
Ordering Guidance
This test should not be requested for patients who have recently received radioisotopes, therapeutically or diagnostically, because of potential assay interference. The specific waiting period before specimen collection will depend on the isotope administered, the dose given, and the clearance rate in the individual patient. Specimens will be screened for radioactivity prior to analysis. Radioactive specimens received in the laboratory will be held 1 week and assayed if sufficiently decayed or canceled if radioactivity remains.
Multiple neurological phenotype-specific autoimmune/paraneoplastic evaluations are available. For more information as well as phenotype-specific testing options, refer to Autoimmune Neurology Test Ordering Guide.
For a list of antibodies performed with each evaluation, see Autoimmune Neurology Antibody Matrix.
Necessary Information
Provide the following information:
-Relevant clinical information
-Ordering provider name, phone number, mailing address, and email address
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Red top
Acceptable: Serum gel
Submission Container/Tube: Plastic vial
Specimen Volume: 2 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Secondary ID
614599Useful For
Evaluating patients with suspected stiff-person syndrome (classical or focal forms, such as stiff-limb or stiff-trunk) and progressive encephalomyelitis with rigidity and myoclonus using serum specimens
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| SPPSI | Stiff-Person/PERM Interp, S | No | Yes |
| AMPHS | Amphiphysin Ab, S | No | Yes |
| DPPCS | DPPX Ab CBA, S | No | Yes |
| GD65S | GAD65 Ab Assay, S | Yes | Yes |
| GLYCS | Glycine Alpha1 LCBA, S | Yes | Yes |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| AMIBS | Amphiphysin Immunoblot, S | No | No |
| DPPTS | DPPX Ab IFA Titer, S | No | No |
| APHTS | Amphiphysin Ab Titer, S | No | No |
Testing Algorithm
If the indirect immunofluorescence assay (IFA) pattern suggests amphiphysin antibody, then amphiphysin immunoblot and amphiphysin IFA titer will be performed at an additional charge.
If the dipeptidyl-peptidase-like protein-6 (DPPX) cell bound assay is positive, then DPPX antibody IFA titer will be performed at an additional charge.
Method Name
AMPHS, APHTS, DPPTS: Indirect Immunofluorescence Assay (IFA)
AMIBS: Immunoblot
DPPCS: Cell-Binding Assay
GD65S: Radioimmunoassay (RIA)
GLYCS: Live Cell-Binding Assay
SPPSI: Medical Interpretation
Reporting Name
Stiff-Person/PERM Eval, SSpecimen Type
SerumSpecimen Minimum Volume
1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 28 days | |
| Frozen | 28 days | ||
| Ambient | 72 hours |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
Reference Values
|
Test ID |
Reporting Name |
Methodology* |
Reference Value |
|
SPPSI |
Stiff-Person/PERM Interp, S |
Medical interpretation |
N/A |
|
AMPHS |
Amphiphysin Ab, S |
IFA |
Negative |
|
DPPCS |
DPPX Ab CBA, S |
CBA |
Negative |
|
GD65S |
GAD65 Ab Assay, S |
RIA |
≤0.02 nmol/L |
|
GLYCS |
Glycine Alpha1 LCBA, S |
LCBA |
Negative |
Reflex Information
|
Test ID |
Reporting Name |
Methodology |
Reference Value |
|
AMIBS |
Amphiphysin Immunoblot, S |
IB |
Negative |
|
APHTS |
Amphiphysin Ab Titer, S |
IFA |
<1:240 |
|
DPPTS |
DPPX Ab IFA Titer, S |
IFA |
<1:240 |
*Methodology abbreviations
Indirect Immunofluorescence Assay (IFA)
Cell-Binding Assay (CBA)
Radioimmunoassay (RIA)
Live Cell-Binding Assay (LCBA)
Immunoblot (IB)
Neuron-restricted patterns of IgG staining that do not fulfill criteria for amphiphysin antibody may be reported as "unclassified antineuronal IgG." Complex patterns that include nonneuronal elements may be reported as "uninterpretable."
Day(s) Performed
Profile tests: Monday through Sunday; Reflex tests: Varies
Report Available
5 to 10 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
86255 x 2
86341
0431U
84182 (if appropriate)
86256 (if appropriate)
86256 (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| SPPS | Stiff-Person/PERM Eval, S | 94701-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 81722 | Amphiphysin Ab, S | 72327-0 |
| 81596 | GAD65 Ab Assay, S | 30347-9 |
| 64933 | DPPX Ab CBA, S | 94676-4 |
| 606972 | Glycine Alpha1 LCBA, S | 96496-5 |
| 614601 | Stiff-Person/PERM Interp, S | 69048-7 |
| 618910 | IFA Notes | 48767-8 |
Forms
If not ordering electronically, complete, print, and send a Neurology Specialty Testing Client Test Request (T732) with the specimen.